Favoreel H W, Nauwynck H J, Pensaert M B
Faculty of Veterinary Medicine, University of Ghent, Ghent, B-9000, Belgium.
Virology. 1999 Jun 20;259(1):141-7. doi: 10.1006/viro.1999.9749.
Pseudorabies virus (PrV) glycoprotein gE is a nonessential glycoprotein involved in virulence and spread of the virus. It also has an important, yet unknown, function during antibody-induced capping of viral glycoproteins on the plasma membrane of PrV-infected swine kidney cells. In the present study, it was shown, by the use of a PrV strain expressing a truncated gE glycoprotein, that the cytoplasmic tail of gE is of significant importance for viral glycoprotein capping to occur. In addition, using PrV strains carrying point mutations in the cytoplasmic tail of gE, it was demonstrated that two tyrosine-based motifs are very important for correct functioning of gE during viral glycoprotein capping. Furthermore it was shown that genistein and tyrphostin, two tyrosine kinase activity inhibitors, inhibit viral glycoprotein capping in a concentration-dependent manner. In conclusion, it can be stated that efficient antibody-induced viral glycoprotein capping requires the presence of two YxxL sequences in the cytoplasmic tail of glycoprotein gE, as well as the activation of a tyrosine phosphorylation signal transduction pathway.
伪狂犬病病毒（PrV）糖蛋白gE是一种非必需糖蛋白，参与病毒的毒力和传播。在抗体诱导的PrV感染的猪肾细胞质膜上病毒糖蛋白封帽过程中，它也具有重要但未知的功能。在本研究中，通过使用表达截短gE糖蛋白的PrV毒株表明，gE的细胞质尾巴对于病毒糖蛋白封帽的发生至关重要。此外，利用在gE细胞质尾巴中携带点突变的PrV毒株证明，两个基于酪氨酸的基序对于病毒糖蛋白封帽过程中gE的正确功能非常重要。此外还表明，两种酪氨酸激酶活性抑制剂染料木黄酮和 tyrphostin以浓度依赖的方式抑制病毒糖蛋白封帽。总之，可以说有效的抗体诱导的病毒糖蛋白封帽需要糖蛋白gE细胞质尾巴中存在两个YxxL序列，以及酪氨酸磷酸化信号转导途径的激活。
