UNC-13对秀丽隐杆线虫UNC-18- syntaxin复合体的调控
Regulation of the UNC-18-Caenorhabditis elegans syntaxin complex by UNC-13.
作者信息
Sassa T, Harada S, Ogawa H, Rand J B, Maruyama I N, Hosono R
机构信息
Department of Nutrition, School of Medicine, Tokushima University, Tokushima 770-0042, Japan.
出版信息
J Neurosci. 1999 Jun 15;19(12):4772-7. doi: 10.1523/JNEUROSCI.19-12-04772.1999.
Abstract
The Caenorhabditis elegans unc-13, unc-18, and unc-64 genes are required for normal synaptic transmission. The UNC-18 protein binds to the unc-64 gene product C. elegans syntaxin (Ce syntaxin). However, it is not clear how this protein complex is regulated. We show that UNC-13 transiently interacts with the UNC-18-Ce syntaxin complex, resulting in rapid displacement of UNC-18 from the complex. Genetic and biochemical evidence is presented that UNC-13 contributes to the modulation of the interaction between UNC-18 and Ce syntaxin.
摘要
秀丽隐杆线虫的unc-13、unc-18和unc-64基因是正常突触传递所必需的。UNC-18蛋白与unc-64基因产物秀丽隐杆线虫 syntaxin（Ce syntaxin）结合。然而，尚不清楚该蛋白复合物是如何被调控的。我们发现UNC-13与UNC-18-Ce syntaxin复合物短暂相互作用，导致UNC-18从复合物中快速解离。我们提供了遗传学和生物化学证据，表明UNC-13有助于调节UNC-18与Ce syntaxin之间的相互作用。
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