Ramírez-Lorca R, Vizuete M L, Venero J L, Revuelta M, Cano J, Ilundáin A A, Echevarría M
Departamento de Fisiología y Biología Animal, Facultad de Farmacia, Universidad de Sevilla, C) Tramontana s/n, E-41012 Sevilla, Spain.
Pflugers Arch. 1999 Jun;438(1):94-100. doi: 10.1007/s004240050884.
Since specific proteins responsible for water transport (aquaporins, AQPs) have been identified in a great variety of tissues, we decided to study the presence of AQP3 in the gastrointestinal tract (GIT) of Wistar rats. Poly(A+) RNA was purified from the mucosa of the stomach, jejunum, ileum and colon, and gross detection of AQP3 mRNA was done by Northern blot analysis. In situ hybridization studies were carried out to precisely localize the distribution of this transcript. Sections of the different tissues were hybridized with @400-bp [35S]riboprobes. The results presented here demonstrate that AQP3 is expressed throughout the GIT, with its expression in the colon and ileum greater than that in the stomach. Immunohistochemistry experiments, using a polyclonal antibody against AQP3, revealed that AQP3 protein is present at the basolateral membrane of the epithelial cells lining the villus tip of the small intestine and colon. The finding of AQP3 in the intestinal epithelia strongly suggests that this protein functions as a pathway for water transport in this epithelium.
由于在多种组织中已鉴定出负责水运输的特定蛋白质（水通道蛋白，AQPs），我们决定研究水通道蛋白3（AQP3）在Wistar大鼠胃肠道（GIT）中的存在情况。从胃、空肠、回肠和结肠的黏膜中纯化出聚腺苷酸（Poly(A+)）RNA，并通过Northern印迹分析对AQP3 mRNA进行总体检测。进行原位杂交研究以精确确定该转录本的分布。用400 bp的[35S]核糖探针与不同组织的切片进行杂交。此处呈现的结果表明，AQP3在整个胃肠道中均有表达，其在结肠和回肠中的表达高于胃中的表达。使用针对AQP3的多克隆抗体进行的免疫组织化学实验显示，AQP3蛋白存在于小肠和结肠绒毛尖端内衬上皮细胞的基底外侧膜上。在肠上皮中发现AQP3强烈表明该蛋白在这种上皮中作为水运输的途径发挥作用。
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