Kobayashi H, Sun G W, Terao T
Department of Obstetrics and Gynecology, Hamamatsu University School of Medicine, Handacho 3600, Hamamatsu, Shizuoka, 431-3192, Japan.
Cell Tissue Res. 1999 Jun;296(3):587-97. doi: 10.1007/s004410051320.
The direct interaction of hyaluronic acid (HA) and heavy chain (HC) of the inter-alpha-trypsin inhibitor (IalphaI) family plays a critical role in the organization and stabilization of the extracellular matrix. The aim of the present investigation was to elucidate the distribution of the IalphaI HC and HA in adult mouse tissues. An immunohistochemical method using a rabbit polyclonal antibody raised against mouse IalphaI heavy-chain peptide and a specific probe for HA (biotinylated HA-binding protein) was used to demonstrate an immunolocalization of IalphaI HC and HA. Distribution and localization of HA was of three types, namely, colocalization with IalphaI HC itself (cartilaginous tissue and ovary), localization around IalphaI HC immunostaining (lung, intestine and skeletal muscle), and localization at a small distance from IalphaI HC or a different distribution pattern (brain, liver, skin and kidney). These results indicate that IalphaI HC could function as an HA-rich matrix stabilizer on the cells of cartilage and maturing ovary, in which IalphaI HC shows colocalization with its predominant ligand, HA.
透明质酸（HA）与α-胰蛋白酶抑制剂（IαI）家族的重链（HC）之间的直接相互作用在细胞外基质的组织和稳定中起着关键作用。本研究的目的是阐明IαI HC和HA在成年小鼠组织中的分布。使用针对小鼠IαI重链肽产生的兔多克隆抗体和HA特异性探针（生物素化HA结合蛋白）的免疫组织化学方法来证明IαI HC和HA的免疫定位。HA的分布和定位有三种类型，即与IαI HC本身共定位（软骨组织和卵巢）、在IαI HC免疫染色周围定位（肺、肠和骨骼肌）以及与IαI HC有小距离的定位或不同的分布模式（脑、肝、皮肤和肾）。这些结果表明，IαI HC可以作为软骨细胞和成熟卵巢细胞上富含HA的基质稳定剂，其中IαI HC与其主要配体HA共定位。
