Scarpa S, Giuffrida A, Fazi M, Coletti A, Palumbo C, Pass H I, Procopio A, Modesti A
Dipartimento Medicina Sperimentale e Patologia, I-00161 Roma, Italy.
Int J Mol Med. 1999 Jul;4(1):67-71. doi: 10.3892/ijmm.4.1.67.
Three human pleural malignant mesothelioma cell cultures (MM) of epithelioid (E1), fibrous (F1) and byphasic (B1) histotype were studied in their synthesis of the extracellular matrix (ECM) components laminin (LM), fibronectin (FN), type IV collagen (cIV), and in their chemotactic and haptotactic migration towards the ECM produced proteins. MM-B1 showed the highest FN synthesis and release; MM-E1 produced the highest quantity of basement membrane constituents LM and cIV; MM-F1 weakly produced and released FN, LM and cIV. MM-B1 had the highest chemotactic and haptotactic motility, MM-F1 migrated toward the lowest concentration of LM while had reduced chemotactic activity toward FN and cIV; MM-E1 had the lowest migratory activity toward each ECM substrate. We demonstrated that three MM of different histotype are characterized by different ECM production and that these differences determine a variable ability of each MM to spread and migrate towards ECM substrates.
研究了三种人胸膜恶性间皮瘤细胞培养物（MM），其组织学类型分别为上皮样（E1）、纤维样（F1）和双向型（B1），观察它们在细胞外基质（ECM）成分层粘连蛋白（LM）、纤连蛋白（FN）、IV型胶原（cIV）合成方面的情况，以及它们对ECM产生的蛋白质的趋化性和趋触性迁移。MM-B1显示出最高的FN合成与释放；MM-E1产生的基底膜成分LM和cIV数量最多；MM-F1产生并释放的FN、LM和cIV较少。MM-B1具有最高的趋化性和趋触性运动能力，MM-F1向最低浓度的LM迁移，而对FN和cIV的趋化活性降低；MM-E1对每种ECM底物的迁移活性最低。我们证明，三种不同组织学类型的MM具有不同的ECM产生特征，并且这些差异决定了每种MM向ECM底物扩散和迁移的不同能力。
Zotero 插件