石杉碱甲对基底核大细胞部损伤所致空间工作记忆缺陷的影响。

Effects of huperzine A on nucleus basalis magnocellularis lesion-induced spatial working memory deficit.

作者信息

Xiong Z Q, Cheng D H, Tang X C

机构信息

State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, China.

出版信息

Zhongguo Yao Li Xue Bao. 1998 Mar;19(2):128-32.

PMID:10374634

Abstract

AIM

To study the effects of huperzine A on nucleus basalis magnocellularis (NBM) lesion-induced spatial working memory impairment.

METHODS

A delayed-non-match-to-sample radial arm maze task was used to study spatial working memory. The choline acetyltransferase (ChAT) activity was determined by the conversion of [3H]acetyl-CoA to [3H]ACh.

RESULTS

Unilateral NBM lesion by kainic acid 0.02 mumol impaired rat's ability to perform this working memory task as evidenced by fewer correct choices after different delay intervals and more total errors to complete the task. This behavioral impairment associated with a decrease in the activity of ChAT by about 40% in the ipsilateral cerebral cortex. Huperzine A (0.2 mg.kg-1 i.p. 30 min before testing) ameliorated this spatial working memory impairment. Physostigmine (0.2-0.3 mg.kg-1 i.p. 20 min before testing) also attenuated the NBM lesion-induced memory deficit.

CONCLUSION

The integrity of NBM is critical for spatial working memory processing, and this working memory impairment induced by NBM lesion can be ameliorated by huperzine A and physostigmine.

摘要

目的

研究石杉碱甲对基底核大细胞部（NBM）损伤所致空间工作记忆障碍的影响。

方法

采用延迟非匹配样本放射状臂迷宫任务研究空间工作记忆。通过[3H]乙酰辅酶A转化为[3H]乙酰胆碱来测定胆碱乙酰转移酶（ChAT）活性。

结果

0.02 μmol的 kainic acid单侧损伤NBM会损害大鼠执行该工作记忆任务的能力，不同延迟间隔后正确选择次数减少以及完成任务的总错误次数增加即可证明。这种行为损伤与同侧大脑皮质中ChAT活性降低约40%相关。石杉碱甲（测试前30分钟腹腔注射0.2 mg·kg-1）改善了这种空间工作记忆障碍。毒扁豆碱（测试前20分钟腹腔注射0.2 - 0.3 mg·kg-1）也减轻了NBM损伤所致的记忆缺陷。