去甲肾上腺素和异戊烯基腺苷对豚鼠离体心室肌细胞钠/钙交换电流的影响。
Effects of norepinephrine and isopentenyladenosine on Na+/Ca2+ exchange currents in isolated guinea pig ventricular myocytes.
作者信息
Dun W, Wu B W, Zhao R R
机构信息
Department of Physiology, Shanxi Medical University, Taiyuan, China.
出版信息
Zhongguo Yao Li Xue Bao. 1998 Mar;19(2):141-4.
AIM
To study the effects of norepinephrine (NE) and isopentenyladenosine (Iso) on Na+/Ca2+ exchange currents and the receptor mechanism.
METHODS
The quasi-steady state current-voltage relationship from the isolated guinea pig ventricular myocytes was measured using whole-cell voltage-clamp techniques with a ramp pulse protocol.
RESULTS
At potential of +50 mV, NE 0.005, 0.05, and 5 mumol.L-1 increased the Ni(2+)-sensitive current by 29% +/- 9%, 72% +/- 11%, and 124% +/- 31.4%, respectively; Iso 1.5, 150, and 1500 nmol.L-1 caused increases in the Ni(2+)-sensitive current by 2.8% +/- 2.8%, 56% +/- 13%, and 102% +/- 12%, respectively. Propranolol 10 mumol.L-1 completely inhibited the current changes induced by NE and Iso while phentolamine 50 mumol.L-1 showed no effects.
CONCLUSION
NE and Iso increased the Na+/Ca2+ exchange currents via stimulation of cardiac beta-adrenoceptor.
目的
研究去甲肾上腺素（NE）和异戊烯基腺苷（Iso）对钠/钙交换电流及受体机制的影响。
方法
采用全细胞膜片钳技术结合斜坡脉冲方案，测量分离的豚鼠心室肌细胞的准稳态电流-电压关系。
结果
在+50 mV电位时，0.005、0.05和5 μmol·L-1的NE分别使镍（2+）敏感电流增加29%±9%、72%±11%和124%±31.4%；1.5、150和1500 nmol·L-1的Iso分别使镍（2+）敏感电流增加2.8%±2.8%、56%±13%和102%±12%。10 μmol·L-1的普萘洛尔完全抑制NE和Iso诱导的电流变化，而50 μmol·L-1的酚妥拉明无作用。
结论
NE和Iso通过刺激心脏β-肾上腺素能受体增加钠/钙交换电流。
Zotero 插件