慢性粒细胞白血病及相关疾病中的所有类型和慢性髓性白血病类型的BCR/ABL mRNA转录本

ALL- and CML-type BCR/ABL mRNA transcripts in chronic myelogenous leukemia and related disorders.

作者信息

Yokohama A, Karasawa M, Okamoto K, Sakai H, Naruse T

机构信息

Third Department of Internal Medicine, Gunma University School of Medicine, Maebashi, Japan.

出版信息

Leuk Res. 1999 May;23(5):477-81. doi: 10.1016/s0145-2126(99)00035-1.

DOI:10.1016/s0145-2126(99)00035-1

PMID:10374861

Abstract

Using the reverse transcription polymerase chain reaction, we investigated acute lymphoid leukemia (ALL)-type, and chronic myelogenous leukemia (CML)-type BCR/ABL mRNA expression in a total of 66 patients with chronic myeloproliferative disorder (CMPD). Thirty-six of 37 patients with CML were positive for CML-type mRNA. Thirteen of the 25 CML had ALL-type mRNA expression. The patients with ET, PV, MF, and CMML did not have any detectable BCR/ABL expression. The most remarkable finding was that two patients, a Ph1-positive CML patient and a patient with a presumptive diagnosis of essential thrombocythemia (ET), showed only ALL-type chimeric mRNA expression.

摘要

我们采用逆转录聚合酶链反应，对66例慢性骨髓增殖性疾病（CMPD）患者的急性淋巴细胞白血病（ALL）型和慢性粒细胞白血病（CML）型BCR/ABL mRNA表达进行了研究。37例CML患者中有36例CML型mRNA呈阳性。25例CML患者中有13例有ALL型mRNA表达。真性红细胞增多症（PV）、原发性血小板增多症（ET）、骨髓纤维化（MF）和慢性粒单核细胞白血病（CMML）患者未检测到任何BCR/ABL表达。最显著的发现是，2例患者，1例Ph1阳性CML患者和1例初步诊断为原发性血小板增多症（ET）的患者，仅表现出ALL型嵌合mRNA表达。