染色体核型正常的复发性自然流产患者的蜕膜自然杀伤细胞
Decidual natural killer cells in recurrent spontaneous abortion with normal chromosomal content.
作者信息
Yamamoto T, Takahashi Y, Kase N, Mori H
机构信息
Department of Obstetrics and Gynecology, Teikyo University School of Medicine, Tokyo, Japan.
出版信息
Am J Reprod Immunol. 1999 May;41(5):337-42. doi: 10.1111/j.1600-0897.1999.tb00447.x.
PROBLEM
The maternal local immune responses in unexplained recurrent spontaneous abortion (RSA) are not yet well known. Maternal peripheral and decidual natural killer (NK) cells were evaluated in RSA with normal chromosomal content.
METHOD OF STUDY
Maternal peripheral blood, villous trophoblast, and decidua were taken from 15 normal pregnancies and 9 RSA patients with normal chromosomes. The NK cells in decidual lymphocytes were evaluated by flow cytometry using monoclonal antibodies for CD56, CD16, and CD3.
RESULTS
The percentages of CD56+ CD16- CD3- cells in decidual lymphocytes in RSA were lower than in normal pregnancies (P < 0.002). The CD56+CD16+/CD56+CD16- cells ratio in RSA was higher than in normal pregnancies (P < 0.02).
CONCLUSION
The lower percentages of CD56+CD16-CD3- cells in RSA cases may show an inappropriate accumulation of NK cells in the decidua, and this finding may be a factor involved in RSA.
问题
不明原因复发性自然流产（RSA）中母体局部免疫反应尚不清楚。对染色体含量正常的RSA患者的母体外周血和蜕膜自然杀伤（NK）细胞进行了评估。
研究方法
从15例正常妊娠和9例染色体正常的RSA患者中采集母体外周血、绒毛滋养层和蜕膜。使用针对CD56、CD16和CD3的单克隆抗体，通过流式细胞术评估蜕膜淋巴细胞中的NK细胞。
结果
RSA患者蜕膜淋巴细胞中CD56 + CD16 - CD3 - 细胞的百分比低于正常妊娠（P < 0.002）。RSA患者中CD56 + CD16 + / CD56 + CD16 - 细胞比例高于正常妊娠（P < 0.02）。
结论
RSA病例中CD56 + CD16 - CD3 - 细胞百分比降低可能表明NK细胞在蜕膜中异常积聚，这一发现可能是RSA的一个相关因素。
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