人源外周血单核细胞-重症联合免疫缺陷小鼠对脑膜炎奈瑟菌C群肽模拟物的免疫反应。

Human immune response to a peptide mimic of Neisseria meningitidis serogroup C in hu-PBMC-SCID mice.

作者信息

Hutchins W A, Kieber-Emmons T, Carlone G M, Westerink M A

机构信息

Department of Medicine, Medical College of Ohio, Toledo 43699, USA.

出版信息

Hybridoma. 1999 Apr;18(2):121-9. doi: 10.1089/hyb.1999.18.121.

DOI:10.1089/hyb.1999.18.121

PMID:10380011

Abstract

An anti-idiotype-based peptide mimic vaccine for Neisseria meningitidis serogroup C polysaccharide (MCPS) has been developed and shown to induce a response in mice that is specific, functional, and T-dependent. In this study, the immunogenicity of the MCPS peptide mimic vaccine preparation, as a potential vaccine for use in humans, is shown using the hu-PBMC-SCID mouse model. The human antibody response to the MCPS peptide mimic vaccine is specific and functional as shown by inhibition enzyme-linked immunoadsorbent assay (ELISA) and bactericidal assay. These data support the usefulness of the peptide mimic vaccine strategy for humans.

摘要

一种基于抗独特型的肽模拟疫苗已被开发用于脑膜炎奈瑟菌C群多糖（MCPS），并已证明可在小鼠中诱导特异性、功能性且依赖T细胞的反应。在本研究中，使用人外周血单核细胞-重症联合免疫缺陷（hu-PBMC-SCID）小鼠模型展示了MCPS肽模拟疫苗制剂作为一种潜在的人用疫苗的免疫原性。如抑制酶联免疫吸附测定（ELISA）和杀菌试验所示，人对MCPS肽模拟疫苗的抗体反应具有特异性和功能性。这些数据支持了肽模拟疫苗策略对人类的实用性。