平肠球菌钾/质子反向转运系统缺陷型突变体的分离与特性研究
Isolation and properties of Enterococcus hirae mutants defective in the potassium/proton antiport system.
作者信息
Kakinuma Y, Igarashi K
机构信息
Faculty of Pharmaceutical Sciences, Chiba University, Inage-ku, Chiba 263-8522, Japan.
出版信息
J Bacteriol. 1999 Jul;181(13):4103-5. doi: 10.1128/JB.181.13.4103-4105.1999.
Abstract
A K+/H+ antiporter regulates cytoplasmic pH in Enterococcus hirae growing at alkaline pH. Mutants defective in this antiport activity were alkaline pH sensitive. One mutant, Pop1, lacked both K+/methylamine exchange at pH 9.5 and concomitant acidification of cytoplasmic pH. Pop1 grew well at pHs below 8 but did not at pHs above 9, conditions under which cytoplasmic pH was not fully acidified.
摘要
一种钾离子/氢离子反向转运蛋白可调节在碱性pH条件下生长的希氏肠球菌的细胞质pH。这种反向转运活性有缺陷的突变体对碱性pH敏感。其中一个突变体Pop1在pH 9.5时既缺乏钾离子/甲胺交换，也缺乏伴随的细胞质pH酸化。Pop1在pH低于8时生长良好，但在pH高于9时不能生长，此时细胞质pH未完全酸化。
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