代谢型谷氨酸受体mGluR1 mRNA1一种新的可变剪接形式的鉴定、克隆及表达分析
Identification, cloning and analysis of expression of a new alternatively spliced form of the metabotropic glutamate receptor mGluR1 mRNA1.
作者信息
Soloviev M M, Ciruela F, Chan W Y, McIlhinney R A
机构信息
Medical Research Council, Anatomical Neuropharmacology Unit, Mansfield Road, Oxford OX1 3TH, UK.
出版信息
Biochim Biophys Acta. 1999 Jul 7;1446(1-2):161-6. doi: 10.1016/s0167-4781(99)00083-4.
We have applied quantitative RT-PCR analysis to characterise relative levels of expression of the alternatively spliced mGluR1 mRNAs. This has also allowed us to identify and clone a new alternatively spliced form of the mGluR1 mRNA. The newly identified mGluR1f mRNA is expressed at moderate levels in rat brain, reaching its maximum in cortex. mGluR1f differs from the mGluR1a mRNA by deletion of a 35-bp fragment of the mGluR1a/alpha coding sequence and insertion of an 85-bp fragment, found only in mGluR1b/beta mRNA.
我们应用定量逆转录聚合酶链反应（RT-PCR）分析来表征可变剪接的代谢型谷氨酸受体1（mGluR1）信使核糖核酸（mRNA）的相对表达水平。这也使我们能够鉴定并克隆一种新的mGluR1 mRNA可变剪接形式。新鉴定出的mGluR1f mRNA在大鼠脑中以中等水平表达，在皮层中达到最高值。mGluR1f与mGluR1a mRNA的不同之处在于，mGluR1a/α编码序列缺失了一个35个碱基对的片段，并且插入了一个仅在mGluR1b/β mRNA中发现的85个碱基对的片段。
Zotero 插件