Pacifico F, Liguoro D, Acquaviva R, Formisano S, Consiglio E
Centro di Endocrinologia ed Oncologia Sperimentale G. Salvatore, CNR, Naples, Italy.
Biochimie. 1999 May;81(5):493-6. doi: 10.1016/s0300-9084(99)80100-x.
The ability of asialo-thyroglobulin to bind the thyroid RHL-1 subunit of the asialoglycoprotein receptor has been investigated. Ligand blot assays show that the recombinant carbohydrate recognition domain of the thyroid RHL-1 subunit specifically interacts with rat desialated thyroglobulin. Moreover, RT-PCR and Western blot assays show that TSH deprivation decreases RHL-1 expression in PC C13 thyroid differentiated cells whereas insulin deprivation does not have any effect. The simultaneous absence of both TSH and insulin dramatically decreases the level of RHL-1 expression.
去唾液酸甲状腺球蛋白与去唾液酸糖蛋白受体的甲状腺RHL-1亚基结合的能力已得到研究。配体印迹分析表明，甲状腺RHL-1亚基的重组碳水化合物识别结构域与大鼠去唾液酸化甲状腺球蛋白特异性相互作用。此外，逆转录聚合酶链反应（RT-PCR）和蛋白质免疫印迹分析表明，促甲状腺激素（TSH）缺乏会降低PC C13甲状腺分化细胞中RHL-1的表达，而胰岛素缺乏则没有任何影响。TSH和胰岛素同时缺乏会显著降低RHL-1的表达水平。
