棉鼠（棉尾鼠）自发性胃癌的神经内分泌（ECL细胞）分化
Neuroendocrine (ECL cell) differentiation of spontaneous gastric carcinomas of cotton rats (Sigmodon hispidus).
作者信息
Waldum H L, Rørvik H, Falkmer S, Kawase S
机构信息
Department of Medicine, University Hospital, Faculty of Medicine, Norwegian University of Science and Technology, Trondheim.
出版信息
Lab Anim Sci. 1999 Jun;49(3):241-7.
BACKGROUND AND PURPOSE
Female inbred cotton rats develop adenocarcinomas in the oxyntic mucosa. Since a female preponderance is typical for enterochromaffin-like (ECL) cell tumors, we examined such tumors for ECL cells. Gastrin plays a decisive role in ECL cell tumorigenesis, so blood gastrin concentration and gastric mucosal pH were measured.
METHODS
The stomachs from six female cotton rats (6 to 8 months old) were studied histologically, and at euthanasia, gastric mucosal pH was determined. Euthanasia was performed on 15 other female cotton rats of similar age for determination of blood gastrin values by radioimmunoassay (RIA) and gastric mucosal pH. Rats were classified macroscopically to have normal or thick oxyntic mucosa, with or without tumor.
RESULTS
Among the six cotton rats studied histologically, two 6-month-old rats had normal and two others had thick gastric mucosa, whereas two 8-month-old rats had thick mucosa with tumors. The ECL cells were markedly hyperplastic in all rats with thick mucosa, and ECL cells were found in the neoplastic parenchyma. All cotton rats with normal-appearing gastric mucosa had pH <2.5, whereas 14 rats with thick mucosa had pH >3.1 and hypergastrinemia.
CONCLUSIONS
Gastrin may play a major role in ECL cell hyperplasia and, perhaps, in adenocarcinoma genesis.
背景与目的
雌性近交系棉鼠胃体黏膜会发生腺癌。由于肠嗜铬样（ECL）细胞瘤通常以雌性居多，我们对这类肿瘤中的ECL细胞进行了检查。胃泌素在ECL细胞瘤发生过程中起决定性作用，因此测定了血胃泌素浓度和胃黏膜pH值。
方法
对6只6至8月龄雌性棉鼠的胃进行组织学研究，并在安乐死时测定胃黏膜pH值。对另外15只年龄相仿的雌性棉鼠实施安乐死，通过放射免疫分析（RIA）测定血胃泌素值并测定胃黏膜pH值。根据大体观察将大鼠分为胃体黏膜正常或增厚，有无肿瘤的情况。
结果
在接受组织学研究的6只棉鼠中，两只6月龄大鼠胃黏膜正常，另外两只增厚，而两只8月龄大鼠胃黏膜增厚且有肿瘤。所有胃黏膜增厚的大鼠中ECL细胞均显著增生，肿瘤实质中也发现了ECL细胞。所有胃黏膜外观正常的棉鼠胃黏膜pH值均<2.5，而14只胃黏膜增厚的大鼠胃黏膜pH值>3.1且有高胃泌素血症。
结论
胃泌素可能在ECL细胞增生中起主要作用，或许在腺癌发生过程中也起主要作用。
Zotero 插件