Teitelbaum R, Schubert W, Gunther L, Kress Y, Macaluso F, Pollard J W, McMurray D N, Bloom B R
Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
Immunity. 1999 Jun;10(6):641-50. doi: 10.1016/s1074-7613(00)80063-1.
M. tuberculosis accesses the terminal lung and is phagocytosed by alveolar macrophages. Utilizing a mouse intratracheal challenge model, we demonstrate that M. tuberculosis rapidly enters through M cells as well. From there, bacilli are deposited within associated intraepithelial leukocytes and subsequently conveyed to the draining lymph nodes early after infection. Osteopetrotic (Csfm(op)/Csfm(op)) mice, null mutants for macrophage colony-stimulating factor, possess diminished numbers of circulating monocytes and tissue macrophages. Csfm(op)/Csfm(op) mice were highly susceptible to challenge with M. tuberculosis. In contrast to controls, tubercle bacilli were not conveyed to draining lymph nodes early after infection but were instead retained within the mucosa. These results indicate that M cells represent an alternate portal of entry for M. tuberculosis, which may contribute to the rapid development of protective lung immune responses.
结核分枝杆菌进入终末肺并被肺泡巨噬细胞吞噬。利用小鼠气管内攻击模型，我们证明结核分枝杆菌也能迅速通过M细胞进入。从那里，杆菌沉积在相关的上皮内白细胞中，并在感染后早期随后被输送到引流淋巴结。骨质石化（Csfm(op)/Csfm(op)）小鼠是巨噬细胞集落刺激因子的无效突变体，其循环单核细胞和组织巨噬细胞数量减少。Csfm(op)/Csfm(op)小鼠对结核分枝杆菌攻击高度敏感。与对照组相比，结核杆菌在感染后早期未被输送到引流淋巴结，而是保留在粘膜内。这些结果表明，M细胞代表结核分枝杆菌的另一个进入门户，这可能有助于肺部保护性免疫反应的快速发展。
