Buisson-Legendre N, Bernard P, Bobichon H, Emonard H, Schneider C, Maquart F X, Haye B, Hornebeck W
UPRESA 6021 CNRS IFR 53 Biomolécules, Faculté de Médecine et Faculté des Sciences, Université de Reims-Champagne Ardenne, Reims, France.
Biochem Biophys Res Commun. 1999 Jul 14;260(3):634-40. doi: 10.1006/bbrc.1999.0565.
Triggering the ceramide pathway by exogenous treatment with neutral sphingomyelinase (Smase) inhibited human keratinocyte growth rate, while having no influence on cell apoptosis. Increasing the ceramide content of keratinocytes with Smase (100 U/ml) or C6-ceramide (1 microM) enhanced matrix metalloproteinase (MMP)-9 production. On the contrary, levels of MMP-2 secretion were unchanged. The inhibition of keratinocyte growth rate induced by ceramide could be annihilated by a peptide hydroxamate MMP inhibitor or an MMP-9 blocking antibody. In addition, inhibiting MMP-9 activity in control keratinocyte culture was found to stimulate keratinocyte proliferation. These data suggest a pivotal function of MMP-9 in the control of keratinocyte growth.
用中性鞘磷脂酶（Smase）进行外源处理触发神经酰胺途径可抑制人角质形成细胞的生长速率，而对细胞凋亡没有影响。用Smase（100 U/ml）或C6-神经酰胺（1 microM）增加角质形成细胞的神经酰胺含量可增强基质金属蛋白酶（MMP）-9的产生。相反，MMP-2的分泌水平没有变化。神经酰胺诱导的角质形成细胞生长速率抑制可被肽羟基肟酸MMP抑制剂或MMP-9阻断抗体消除。此外，发现在对照角质形成细胞培养物中抑制MMP-9活性可刺激角质形成细胞增殖。这些数据表明MMP-9在角质形成细胞生长控制中起关键作用。
Zotero 插件