[应用双色荧光原位杂交技术对人植入前胚胎卵裂球进行性别鉴定]
[Sex determination of human preimplantation embryo blastomere using dualcolor fluorescence in situ hybridization].
作者信息
Li W, Lu G
机构信息
Human Reproductive Engineering Laboratory, Hunan Medical University, Changsha, 410078 P.R.China.
出版信息
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 1999 Aug;16(4):255-8.
OBJECTIVE
To establish a series of techniques of sex determination of human preimplantation embryo in order that preimplantation genetic diagnosis(PGD) can be used clinically.
METHODS
Use micromanipulation for biopsy of human preimplantation embryo(3-10 cell) and prepare single-cell interphase nuclei sample. Make sex determination of human preimplantation embryos by using dual-color fluorescence in situ hybridization.
RESULTS
The technique of human preimplantation embryo (3-10 cell) biopsy was established preliminarily and the technique of preparing single-cell interphase nuclei sample was set up and stabilized. The sex determination of the blastomere of human preimplantation embryo was successful.
CONCLUSION
This series of techniques can make fast diagnosis (less than 6 hours) with minimal sample(only one cell), with no cell culture, and with high accuracy of sex determination (100%).
目的
建立一系列人类植入前胚胎性别鉴定技术，以便将植入前遗传学诊断（PGD）应用于临床。
方法
采用显微操作技术对人类植入前胚胎（3 - 10细胞期）进行活检，制备单细胞间期核样本。运用双色荧光原位杂交技术对人类植入前胚胎进行性别鉴定。
结果
初步建立了人类植入前胚胎（3 - 10细胞期）活检技术，建立并稳定了单细胞间期核样本制备技术。人类植入前胚胎卵裂球性别鉴定成功。
结论
这一系列技术能够以最少的样本（仅一个细胞）进行快速诊断（少于6小时），无需细胞培养，性别鉴定准确率高（100%）。
Zotero 插件