猴空泡病毒40大T抗原的J结构域在细胞转化中的作用。

The role of the J domain of SV40 large T in cellular transformation.

作者信息

DeCaprio J A

机构信息

Dana-Farber Cancer Institute, Harvard Medical School, 44 Binney Street, Boston, Massachusetts 02115, USA.

出版信息

Biologicals. 1999 Mar;27(1):23-8. doi: 10.1006/biol.1998.0173.

DOI:10.1006/biol.1998.0173

PMID:10441399

Abstract

SV40 large T antigen (TAg)-mediated transformation is dependent on binding to p53 and the retinoblastoma tumor suppressor protein (pRB) and inactivating their growth suppressive functions. Transformation minimally requires three regions of TAg: a C-terminal domain that mediates binding to p53; the LXCXE motif (residues 103-107), necessary for binding to pRB and the related proteins p107 and p130; and an N-terminal domain (residues 1-82) that contains homology to the J domain found in cellular DnaJ/Hsp40 molecular chaperone proteins. We have found that the N-terminal J domain of T Ag cooperates with the LXCXE motif to inactivate the growth suppressive functions of the pRB-related proteins.

摘要

SV40大T抗原（TAg）介导的转化依赖于与p53和视网膜母细胞瘤肿瘤抑制蛋白（pRB）结合并使其生长抑制功能失活。转化至少需要TAg的三个区域：介导与p53结合的C末端结构域；与pRB以及相关蛋白p107和p130结合所必需的LXCXE基序（第103 - 107位氨基酸残基）；以及与细胞DnaJ/Hsp40分子伴侣蛋白中发现的J结构域具有同源性的N末端结构域（第1 - 82位氨基酸残基）。我们发现TAg的N末端J结构域与LXCXE基序协同作用，使pRB相关蛋白的生长抑制功能失活。