Park H O, Sanson A, Herskowitz I
Department of Molecular Genetics, Ohio State University, Columbus, Ohio 43210-1292, USA.
Genes Dev. 1999 Aug 1;13(15):1912-7. doi: 10.1101/gad.13.15.1912.
Yeast cells of different cell type exhibit distinct budding patterns that reflect the organization of the actin cytoskeleton. Bud1p (Rsr1p), a Ras-like GTPase, and Bud2p, a GTPase-activating protein for Bud1p, are essential for proper budding pattern. We show that Bud2p is localized at the presumptive bud site in G(1) cells in all cell types and that this localization is independent of Bud1p. Bud2p subsequently localizes to the mother-bud neck after bud emergence; this localization depends on the integrity of the septins. These observations indicate that Bud2p becomes positioned in G(1) cells by recognizing cell type-specific landmarks at the presumptive bud site.
不同细胞类型的酵母细胞呈现出独特的出芽模式，这些模式反映了肌动蛋白细胞骨架的组织方式。Bud1p（Rsr1p），一种Ras样GTP酶，以及Bud2p，Bud1p的一种GTP酶激活蛋白，对于正确的出芽模式至关重要。我们发现，在所有细胞类型的G(1)期细胞中，Bud2p定位于假定的芽位点，且这种定位不依赖于Bud1p。芽出现后，Bud2p随后定位于母细胞与芽之间的颈部；这种定位取决于隔膜蛋白的完整性。这些观察结果表明，Bud2p通过识别假定芽位点处细胞类型特异性的标志物，在G(1)期细胞中定位。
