Ueda H, Tashiro S, Kojima S, Tanaka K, Eguchi M, Ueda K, Kamada N
Department of Pediatrics, Hiroshima University School of Medicine, Japan.
Int J Hematol. 1999 Jul;70(1):13-9.
Monosomy 7 in isolated bone marrow mononuclear cells and colony forming cells from patients with aplastic anemia who later developed myelodysplastic syndrome/leukemia with monosomy 7 were examined by fluorescence in situ hybridization. Colonies derived from bone marrow mononuclear cells of the aplastic anemia patients consisted of a mixture of cells of normal karyotype and monosomy 7, ranging from 0 to 97.2%. This result suggests that colony forming cells initially had a normal karyotype but then lost chromosome 7 during growth in the semisolid culture. This finding suggests the genetically unstable condition of chromosome 7 in colony forming cells in aplastic anemia patients. This chromosome instability of colony forming cells may lead to malignant transformation.
采用荧光原位杂交技术检测了再生障碍性贫血患者的孤立骨髓单个核细胞和集落形成细胞中的7号染色体单体，这些患者后来发展为伴有7号染色体单体的骨髓增生异常综合征/白血病。再生障碍性贫血患者骨髓单个核细胞衍生的集落由正常核型细胞和7号染色体单体细胞混合组成，比例从0到97.2%不等。这一结果表明，集落形成细胞最初具有正常核型，但在半固体培养生长过程中随后丢失了7号染色体。这一发现提示再生障碍性贫血患者集落形成细胞中7号染色体的遗传不稳定状态。集落形成细胞的这种染色体不稳定性可能导致恶性转化。
