Hemolysin of Myxococcus fulvus NK 35 i. production and isolation.

Myxococcus fulvus NK 35 has been shown to produce a soluble hemolysin which lysed rabbit, human, horse, and sheep erythrocytes. A medium (Varghese's medium) was devised in which a maximum of hemolysin was produced in 6 days at 28 degrees C under static conditions. The lysin was precipitated by complete saturation of the culture filtrate with ammonium sulphate, followed by dialysis against saline. Other enzyme systems were destroyed by heating at 100 degrees C. Further purification was achieved by passing through a Sephadex G-25 column, giving a single peak with 0.01 M of phosphate buffer, pH 6.6. Like the hemolysin of Pseudomonas aeruginosa, this hemolysin is non-proteinic and withstands 100 degrees C for 30 minutes.