Gratama J W, Bolhuis R L, Van 't Veer M B
Department of Clinical and Tumor Immunology, University Hospital, Daniel den Hoed Cancer Center, Rotterdam, The Netherlands.
Clin Lab Haematol. 1999 Jun;21(3):155-60. doi: 10.1046/j.1365-2257.1999.00214.x.
Immunophenotyping of haematological malignancies has developed as a clinically valuable but technically complicated diagnostic procedure. It involves a variety of methodological features, in-process strategic judgements and an extensive knowledge of clinical, morphological and other laboratory features of the disease processes under study. We discuss the various internal quality control steps necessary to guarantee reliable results with respect to instrument set-up and calibration; sample preparation; selection and validation of monoclonal antibody panels; and flow cytometric data acquisition, analysis and interpretation of results. The quality of the entire procedure is documented by the analysis of representative specimens in the setting of an external quality assurance programme.
血液系统恶性肿瘤的免疫表型分析已发展成为一种具有临床价值但技术复杂的诊断程序。它涉及多种方法学特征、过程中的策略判断以及对所研究疾病过程的临床、形态学和其他实验室特征的广泛了解。我们讨论了为保证仪器设置和校准、样本制备、单克隆抗体组合的选择和验证以及流式细胞术数据采集、分析和结果解释等方面的可靠结果所必需的各种内部质量控制步骤。通过在外部质量保证计划中对代表性标本的分析来记录整个程序的质量。
