Kusuda J, Hirai M, Tanuma R, Hirata M, Hashimoto K
Division of Genetic Resources, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, Japan.
Cytogenet Cell Genet. 1999;85(3-4):248-51. doi: 10.1159/000015303.
The intron-containing genes encoding human and mouse ribosomal protein (r-protein) L27A were cloned and sequenced. The human r-protein L27A gene (RPL27A) shared an identical exon/intron structure with the mouse r-protein 27A gene (Rpl27a). The translational start codon ATG was separated from the main reading frame by the first intron sequence in both genes. An approximately 200-bp sequence upstream of the translational start site of both genes displayed remarkable similarity, and contained the putative promoters lacking canonical TATA, but harbored Sp1 binding sites and a short stretch of pyrimidine cluster, similar to other r-protein genes. Transcriptional regulatory elements, Box-A and GABP, found in the promoters of some other r-protein genes were also conserved in both genes. These structural features were included in the typical CpG island identified in the 5'-end sequences, suggesting that RPL27A/Rpl27a cloned here are authentic and transcriptionally active. Fluorescence in situ hybridization (FISH) analysis localized the mouse intron-containing Rpl27a to chromosome 7E2-F1 syntenic to human chromosome 11p15, where human RPL27A was located.
对编码人及小鼠核糖体蛋白（r蛋白）L27A的含内含子基因进行了克隆和测序。人r蛋白L27A基因（RPL27A）与小鼠r蛋白27A基因（Rpl27a）具有相同的外显子/内含子结构。两个基因的翻译起始密码子ATG均被第一个内含子序列与主要阅读框隔开。两个基因翻译起始位点上游约200bp的序列显示出显著的相似性，并且包含推定的启动子，这些启动子缺乏典型的TATA，但含有Sp1结合位点和一小段嘧啶簇，这与其他r蛋白基因相似。在其他一些r蛋白基因启动子中发现的转录调控元件Box-A和GABP在这两个基因中也保守存在。这些结构特征包含在5'端序列中鉴定出的典型CpG岛中，表明此处克隆的RPL27A/Rpl27a是真实的且具有转录活性。荧光原位杂交（FISH）分析将小鼠含内含子的Rpl27a定位到与人染色体11p15同线的染色体7E2-F1上，人RPL27A也位于该位置。
