Talukder K A, Dutta D K, Albert M J
J Med Microbiol. 1999 Aug;48(8):781-784. doi: 10.1099/00222615-48-8-781.
Pulsed-field gel electrophoresis (PFGE) has been used successfully to discriminate between strains of many different bacterial species. In this study, digestion of bacterial DNA with the restriction endonuclease NotI and PFGE were evaluated for the typing of isolates of Shigella dysenteriae type 1, an important cause of epidemic dysentery. There were 27 isolates from four outbreaks of dysentery, and 44 isolates from endemic dysentery cases and a laboratory culture collection. The epidemic isolates yielded two types each with two subtypes, whereas the endemic isolates and culture collection yielded eight types with numerous subtypes. These findings suggest that S. dysenteriae 1 can be typed by PFGE.
脉冲场凝胶电泳（PFGE）已成功用于区分许多不同细菌物种的菌株。在本研究中，评估了用限制性内切酶NotI消化细菌DNA和PFGE对痢疾志贺菌1型分离株进行分型的情况，痢疾志贺菌1型是流行性痢疾的一个重要病因。有来自4起痢疾暴发的27株分离株，以及来自地方性痢疾病例和实验室培养物保藏库的44株分离株。流行性分离株产生了两种类型，每种类型有两个亚型，而地方性分离株和培养物保藏库产生了八种类型，有许多亚型。这些结果表明，痢疾志贺菌1型可用PFGE进行分型。
