汉族人群中载脂蛋白E等位基因频率按年龄和性别的分布情况。

Population distributions of allele frequency of apolipoprotein E by age and gender in Han Chinese.

作者信息

Zhang J G, Dong X Z, Yang W J, Lu Q, He L

机构信息

Section of Human Genome Project, Shanghai Research Center of Life Sciences, Chinese Academy of Sciences, China.

出版信息

Zhongguo Yao Li Xue Bao. 1999 Mar;20(3):218-22.

PMID:10452095

Abstract

AIM

To study apolipoproteins E (ApoE) allele frequency in Han Chinese based on age and gender from Shanghai metropolitan area.

METHODS

Healthy Han Chinese people (F: 237 and M: 412) were involved in this study. ApoE gene was amplified by PCR using the forward primer: 5'-GGC ACG GCT GTC CAA GGA GCT-3' and reverse primer: 5'-GAT GGC GCT GAG GCC GCG CT-3'. The PCR product was digested directly with 5 units of CfoI and separated by a 20% polyacrylamide nondenaturing gel.

RESULTS

ApoE3 was the commonest allele which accounted for 86.4% of the isoforms, and ApoE2 and ApoE*4 accounted for 6.2% and 7.5%, respectively. The allele and genotype frequencies were in Hardy-Weinberg equilibrium by comparison with that of the corresponding theoretical distribution (P > 0.05).

CONCLUSION

The frequencies of ApoE2, ApoE3, and ApoE*4 were demonstrated in the normal Chinese population.

摘要

目的

基于上海市区汉族人群的年龄和性别研究载脂蛋白E（ApoE）等位基因频率。

方法

本研究纳入健康汉族人群（女性237例，男性412例）。使用正向引物5'-GGC ACG GCT GTC CAA GGA GCT-3'和反向引物5'-GAT GGC GCT GAG GCC GCG CT-3'通过聚合酶链反应（PCR）扩增ApoE基因。PCR产物直接用5单位的CfoI酶切，并用20%的聚丙烯酰胺非变性凝胶进行分离。