一氧化氮和白细胞介素-10对小鼠肺泡巨噬细胞中肿瘤坏死因子α、白细胞介素-1β和白细胞介素-6产生的抑制作用。

Inhibitory effects of nitric oxide and interleukin-10 on production of tumor necrosis factor alpha, interleukin-1 beta, and interleukin-6 in mouse alveolar macrophages.

作者信息

Qiu H B, Chen D C, Pan J Q, Liu D W, Ma S

机构信息

Department of Critical Care Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, China.

出版信息

Zhongguo Yao Li Xue Bao. 1999 Mar;20(3):271-5.

PMID:10452106

Abstract

AIM

To observe the effects of nitric oxide and interleukin-10 (IL-10) on inflammatory reaction in mouse alveolar macrophages (AM).

METHODS

AM from mice were stimulated by lipopolysaccharides (LPS) 10 mg.L-1 and nitric-oxide synthase inhibitor, S-methylisothiorea sulfate (SMT) or nitric-oxide donor, S-nitroso-N-acetyl-D, L-penicillamine (SNAP). The production of tumor necrosis factor alpha (TNF alpha), IL-1 beta, IL-6, and IL-10 by AM were measured by ELISA.

RESULTS

After LPS-stimulation, TNF alpha, IL-1 beta, and IL-6 peaked at 6, 12, and 24 h, respectively by AM. SMT inhibited LPS-induced nitric oxide release and increased IL-1 beta and IL-6 secretions in AM, but the TNF alpha levels remained unchanged. SNAP had inhibitory effects on IL-1 beta and IL-6 secretions in a concentration-dependent manner, but exerted no effect on TNF alpha release. TNF alpha, IL-1 beta, and IL-6 secretions were inhibited by recombinant IL-10, but the cytokines release was upregulated by anti-IL-10 monoclonal antibody.

CONCLUSION

Both endogenous and exogenous nitric oxide and IL-10 had inhibitory effects on the LPS-induced TNF alpha, IL-1 beta, and IL-6 secretions in mouse AM.

摘要

目的

观察一氧化氮和白细胞介素-10（IL-10）对小鼠肺泡巨噬细胞（AM）炎症反应的影响。

方法

用10mg.L-1脂多糖（LPS）、一氧化氮合酶抑制剂硫酸S-甲基异硫脲（SMT）或一氧化氮供体S-亚硝基-N-乙酰-D，L-青霉胺（SNAP）刺激小鼠的AM。采用酶联免疫吸附测定法检测AM产生的肿瘤坏死因子α（TNFα）、IL-1β、IL-6和IL-10。

结果

LPS刺激后，AM产生的TNFα、IL-1β和IL-6分别在6、12和24小时达到峰值。SMT抑制LPS诱导的一氧化氮释放，并增加AM中IL-1β和IL-6的分泌，但TNFα水平保持不变。SNAP以浓度依赖的方式抑制IL-1β和IL-6的分泌，但对TNFα释放无影响。重组IL-10抑制TNFα、IL-1β和IL-6的分泌，但抗IL-10单克隆抗体上调细胞因子的释放。