白细胞介素-10和天花粉蛋白抑制抗原处理细胞的表面分子表达及T细胞增殖。

IL-10 and trichosanthin inhibited surface molecule expression of antigen processing cells and T-cell proliferation.

作者信息

Fan Z S, Ma B L

机构信息

Shanghai Institute of Immunology, Shanghai Second Medical University, China.

出版信息

Zhongguo Yao Li Xue Bao. 1999 Apr;20(4):353-7.

PMID:10452124

Abstract

AIM

To study immunoinhibitory effects and preliminary mechanism of IL-10 and trichosanthin.

METHODS

Surface molecule expression on antigen processing cells (APC) was stained with fluorescence and analyzed by FACScan. B7-1 mRNA expression was detected with nested RT-PCR.

RESULTS

IL-10 2 mg.L-1 and trichosanthin 10 mg.L-1 inhibited B7-1 molecule expression. By contrast, they had not the same effects on ICAM-1. IL-10 and trichosanthin down-regulated LFA-1 expression, but had no regulatory effect on CD40. IL-10 and trichosanthin dramatically inhibited T-cell proliferation and IL-2 production. B7-1 mRNA expression was undetectable in APC treated with IL-10 and trichosanthin.

CONCLUSION

IL-10 and trichosanthin inhibit surface molecule expression on APC. They exert multiple immunoinhibitory effects.

摘要

目的

研究白细胞介素-10（IL-10）和天花粉蛋白的免疫抑制作用及其初步机制。

方法

用荧光标记抗原递呈细胞（APC）表面分子表达，并用流式细胞仪进行分析。采用巢式逆转录聚合酶链反应（nested RT-PCR）检测B7-1 mRNA表达。

结果

IL-10 2 mg.L-1和天花粉蛋白10 mg.L-1可抑制B7-1分子表达。相比之下，它们对细胞间黏附分子-1（ICAM-1）的作用不同。IL-10和天花粉蛋白下调淋巴细胞功能相关抗原-1（LFA-1）表达，但对CD40无调节作用。IL-10和天花粉蛋白显著抑制T细胞增殖和白细胞介素-2（IL-2）产生。在用IL-10和天花粉蛋白处理的APC中未检测到B7-1 mRNA表达。