Grando D, Sullivan L J, Flexman J P, Watson M W, Andrew J H
Department of Microbiology, St. Vincent's Hospital, Melbourne, Australia.
Clin Infect Dis. 1999 May;28(5):1156-8. doi: 10.1086/514756.
Bartonella henselae was recovered from the conjunctival scraping of a 38-year-old woman who presented with a 2-week history of tender preauricular lymphadenopathy and a 1-day history of a red left eye. Dry adherent colonies were observed on agar plates at 21 days of incubation, and the isolate was identified through conventional and molecular tests. Polymerase chain reaction (PCR) amplification of a specific region of the 16S rRNA gene and confirmation by a separate PCR reaction with hybridization of the product with a B. henselae-specific probe confirmed the isolate as B. henselae. This is the first reported isolation of the causative agent of cat scratch disease from ocular tissue in a patient with Parinaud's oculoglandular syndrome.
从一名38岁女性的结膜刮片中分离出汉赛巴尔通体,该患者有2周的耳前淋巴结压痛病史和左眼发红1天的病史。孵育21天时在琼脂平板上观察到干燥的附着菌落,并通过传统和分子检测对分离株进行鉴定。对16S rRNA基因的特定区域进行聚合酶链反应(PCR)扩增,并通过单独的PCR反应进行确认,该反应产物与汉赛巴尔通体特异性探针杂交,证实该分离株为汉赛巴尔通体。这是首次报道在患有帕里诺眼腺综合征的患者的眼部组织中分离出猫抓病的病原体。