Matatov R, Goldhar J, Skutelsky E, Sechter I, Perry R, Podschun R, Sahly H, Thankavel K, Abraham S N, Ofek I
Department of Human Microbiology, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, Israel.
FEMS Microbiol Lett. 1999 Oct 1;179(1):123-30. doi: 10.1111/j.1574-6968.1999.tb08717.x.
We screened phase variants of Klebsiella pneumoniae isolates for the expression of capsule and type 1 fimbriae and found that all of the 22 blood isolates were encapsulated and did not express type 1 fimbriae while 10 of 11 urinary tract isolates expressed type 1 fimbriae but were unencapsulated. Phase variants from selected isolates were found to be either unencapsulated and fimbriated or lacked both structures. Variants expressing both structures were not detected. Fimbrial subunits FimH and FimA were localized in the periplasmic space of the parent strain and on the surface of the unencapsulated variants. The results suggest that capsule formation impedes assembly of pre-formed fimbrial subunits on the bacterial surface.
我们筛选了肺炎克雷伯菌分离株的相变变体以检测荚膜和1型菌毛的表达情况，发现22株血液分离株均有荚膜且不表达1型菌毛，而11株泌尿道分离株中有10株表达1型菌毛但无荚膜。从选定分离株中获得的相变变体要么无荚膜且有菌毛，要么两者结构均缺失。未检测到同时表达两种结构的变体。菌毛亚基FimH和FimA定位于亲本菌株的周质空间以及无荚膜变体的表面。结果表明，荚膜形成会阻碍预先形成的菌毛亚基在细菌表面的组装。
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