Ivanovskaia N P, Osin N S, Khramov E N, Zlobin V N
Vestn Ross Akad Med Nauk. 1999(8):33-8.
The paper shows that the luminescence cytochemical technique can be used for identification of microorganisms and microbiological synthesis products. The method is based on the interaction of specific fluorescence probes (ANS, terbium ions, and beta-diketonate complexes of europium, as well as metal-containing porphyrines) with major microbial intracellular components and toxins. Unlike classical microbiological, immunochemical or biochemical methods of detection, the proposed method has a reasonable versatility, specificity, sensitivity, rapid action, and possible automation.
该论文表明,发光细胞化学技术可用于微生物及微生物合成产物的鉴定。该方法基于特定荧光探针(ANS、铽离子、铕的β-二酮配合物以及含金属卟啉)与主要微生物细胞内成分及毒素的相互作用。与传统的微生物学、免疫化学或生物化学检测方法不同,所提出的方法具有合理的通用性、特异性、灵敏度、快速作用以及可能实现自动化的特点。