Yang M, May W S, Ito T
Sealy Center for Oncology and Hematology, Department of Internal Medicine, The University of Texas Medical Branch, Galveston, Texas 77555-1048, USA.
J Biol Chem. 1999 Sep 24;274(39):27399-406. doi: 10.1074/jbc.274.39.27399.
We have cloned and characterized a novel zinc finger protein, termed JAZ. JAZ contains four C(2)H(2)-type zinc finger motifs that are connected by long (28-38) amino acid linker sequences. JAZ is expressed in all tissues tested and localizes in the nucleus, primarily the nucleolus. JAZ preferentially binds to double-stranded (ds) RNA or RNA/DNA hybrids rather than DNA. Mutation of individual zinc finger motifs reveals that the zinc finger domains are not only essential for dsRNA binding but are also required for its nucleolar localization, which demonstrates a complex trafficking mechanism dependent on the nucleic acid-binding capability of the protein. Furthermore, forced expression of JAZ potently induces apoptosis in murine fibroblast cells. Thus, JAZ may belong to a class of zinc finger proteins that features dsRNA binding and may regulate cell growth via the unique dsRNA binding properties.
我们克隆并鉴定了一种名为JAZ的新型锌指蛋白。JAZ含有四个C(2)H(2)型锌指基序，这些基序由长（28 - 38个）氨基酸连接序列相连。JAZ在所有测试组织中均有表达，并定位于细胞核，主要是核仁。JAZ优先结合双链（ds）RNA或RNA/DNA杂交体而非DNA。单个锌指基序的突变表明，锌指结构域不仅对dsRNA结合至关重要，而且对其核仁定位也是必需的，这表明存在一种依赖于该蛋白质核酸结合能力的复杂转运机制。此外，JAZ的强制表达能有效诱导小鼠成纤维细胞凋亡。因此，JAZ可能属于一类具有dsRNA结合特性的锌指蛋白，并可能通过独特的dsRNA结合特性调节细胞生长。
