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雀形目鸟类金丝雀视网膜中的视觉色素和油滴:显微分光光度法和视蛋白序列

Visual pigments and oil droplets in the retina of a passerine bird, the canary Serinus canaria: microspectrophotometry and opsin sequences.

作者信息

Das D, Wilkie S E, Hunt D M, Bowmaker J K

机构信息

Department of Visual Science, University College London, UK.

出版信息

Vision Res. 1999 Aug;39(17):2801-15. doi: 10.1016/s0042-6989(99)00023-1.

DOI:10.1016/s0042-6989(99)00023-1
PMID:10492811
Abstract

The visual receptors of the passeriform bird Serinus canaria, the canary, have been examined microspectrophotometrically and the sequences of the opsins determined. Rods have a maximum absorbance (lambda max) at 506 nm. Four spectral classes of single cone are present: long-wave-sensitive (LWS) containing a photopigment with lambda max at 569 nm, middle-wave-sensitive (MWS) with lambda max at 505 nm, short-wave-sensitive (SWS) with lambda max at 442 nm, and ultraviolet-sensitive (UVS) with lambda max at about 366 nm. Double cones possess the 569-nm pigment in both members. Typical combinations of photopigment and oil droplet occur in most cone classes. An ambiguity exists in the oil droplet of the single LWS cones. In some birds, LWS cones are paired with an R-type droplet, whereas in the majority of canaries the LWS pigment is paired with a droplet similar to the P-type of double cones. Mechanisms of spectral tuning within each opsin class are discussed.

摘要

已采用显微分光光度法对雀形目鸟类金丝雀(Serinus canaria)的视觉感受器进行了检测,并确定了视蛋白的序列。视杆细胞在506纳米处有最大吸光度(λmax)。存在四种光谱类型的单锥细胞:长波敏感型(LWS),其含有的光色素在569纳米处有λmax;中波敏感型(MWS),λmax在505纳米处;短波敏感型(SWS),λmax在442纳米处;紫外敏感型(UVS),λmax在约366纳米处。双锥细胞的两个成员均含有569纳米的色素。在大多数锥细胞类型中,光色素和油滴存在典型组合。单LWS锥细胞的油滴存在一个模糊性问题。在一些鸟类中,LWS锥细胞与R型油滴配对,而在大多数金丝雀中,LWS色素与类似于双锥细胞P型的油滴配对。文中讨论了每个视蛋白类别内的光谱调谐机制。

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