RMPs：重组/复制介导蛋白。

RMPs: recombination/replication mediator proteins.

作者信息

Beernink H T, Morrical S W

机构信息

Dept of Biochemistry and Center for X-ray Crystallography, The University of Vermont College of Medicine, Burlington, VT 05405-0068, USA.

出版信息

Trends Biochem Sci. 1999 Oct;24(10):385-9. doi: 10.1016/s0968-0004(99)01451-6.

DOI:10.1016/s0968-0004(99)01451-6

PMID:10500302

Abstract

Enzymes for DNA replication and recombination need to gain access to single-stranded DNA (ssDNA) but ssDNA-binding proteins (SSBs) present an obstacle to the formation of enzyme-ssDNA replication and recombination complexes. A specialized class of SSBs, which we designate as recombination/replication mediator proteins (RMPs), promotes enzyme- ssDNA assembly by overcoming SSB inhibition. RMPs exhibit strong conservation of function across divergent species, and display species-specific interactions with SSB and enzymes to neutralize the SSB barrier to enzyme-ssDNA assembly.

摘要

用于DNA复制和重组的酶需要接触单链DNA（ssDNA），但单链DNA结合蛋白（SSB）会阻碍酶-ssDNA复制和重组复合物的形成。一类特殊的SSB，我们将其命名为重组/复制介导蛋白（RMP），通过克服SSB的抑制作用来促进酶-ssDNA组装。RMP在不同物种间表现出强大的功能保守性，并与SSB和酶呈现物种特异性相互作用，以消除SSB对酶-ssDNA组装的障碍。