Costa J J, Harris A G, Delano F A, Zweifach B W, Schmid-Schönbein G W
Institute for Biomedical Engineering and Department of Bioengineering, University of California, San Diego, La Jolla 92093-0412, USA.
Microcirculation. 1999 Sep;6(3):237-44.
The objective of this study was to explore the degree of parenchymal cell injury after mast cell degranulation by application of compound 48/80 (CMP 48/80) in the absence of adherent leukocytes in the rat mesentery.
Rats were rendered leukopenic by injection of an antibody against leukocytes, and the mesentery was superfused with CMP 48/80 during intravital microscopy. The extent of cell injury was determined using a fluorescent cell-viability indicator, propidium iodide (PI). In an additional group, mast cell degranulation with CMP 48/80 was prevented by using the mast cell stabilizer Ketotifen.
After a reduction in the number of circulating leukocytes, mast cell degranulation produced a mild increase in parenchymal cell injury. The injury levels significantly increased when individual regions of the mesentery were compared. Stabilization of the mast cells with Ketotifen reduced the injury to below baseline values.
In the absence of leukocyte adhesion to the endothelium, mast cell degranulation contributes to parenchymal cell injury in the mesentery.
本研究的目的是在大鼠肠系膜中不存在黏附白细胞的情况下，通过应用化合物48/80（CMP 48/80）来探究肥大细胞脱颗粒后实质细胞的损伤程度。
通过注射抗白细胞抗体使大鼠白细胞减少，在活体显微镜检查期间用CMP 48/80对肠系膜进行灌流。使用荧光细胞活力指示剂碘化丙啶（PI）确定细胞损伤程度。在另一组中，使用肥大细胞稳定剂酮替芬防止CMP 48/80引起的肥大细胞脱颗粒。
循环白细胞数量减少后，肥大细胞脱颗粒使实质细胞损伤轻度增加。当比较肠系膜的各个区域时，损伤水平显著增加。用酮替芬使肥大细胞稳定可将损伤降低至基线值以下。
在内皮细胞不存在白细胞黏附的情况下，肥大细胞脱颗粒会导致肠系膜中的实质细胞损伤。
Zotero 插件