Katagiri T, Hirono I, Aoki T
Laboratory of Genetics and Biochemistry, Tokyo University of Fisheries, Konan 4-5-7, Minato-ku, Tokyo 108-8477, Japan.
Immunogenetics. 1999 Oct;50(1-2):43-8. doi: 10.1007/s002510050684.
The amino acid sequences of the human terminal complement components show extensive structural similarity to each other. In this study the C8beta and C9 cDNAs of Japanese flounder, Paralichthys olivaceus, were cloned and analyzed. The derived deduced amino acid sequences of the two terminal components were homologous to those of humans, in that the sequences of both species contained LDL receptor, EGF precursor, and two thrombospondin domains. Japanese flounder C9 was found to have a second thrombospondin region in the C-terminus, similar to that reported for rainbow trout and pufferfish. Moreover, these two complement component cDNAs of Japanese flounder had partial similarity to human perforin. These findings show that Japanese flounder C8beta and C9 have similar structures, which supports the hypothesis that the terminal complement genes originated from the same ancestral gene. Collectively, these features emphasize the strong similarity among the members of the terminal complement family.
人类末端补体成分的氨基酸序列彼此显示出广泛的结构相似性。在本研究中，克隆并分析了日本牙鲆（Paralichthys olivaceus）的C8β和C9 cDNA。这两种末端成分推导的氨基酸序列与人类的同源，因为两个物种的序列都包含低密度脂蛋白受体、表皮生长因子前体和两个血小板反应蛋白结构域。发现日本牙鲆C9在C末端有第二个血小板反应蛋白区域，类似于虹鳟鱼和河豚中报道的那样。此外，日本牙鲆的这两种补体成分cDNA与人类穿孔素具有部分相似性。这些发现表明日本牙鲆C8β和C9具有相似结构，这支持了末端补体基因起源于同一祖先基因的假说。总体而言，这些特征强调了末端补体家族成员之间的高度相似性。
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