莱茵衣藻中的一对反向重复基因编码一种合子特异性蛋白，其表达对紫外线敏感。

A pair of invertedly repeated genes in Chlamydomonas reinhardtii encodes a zygote-specific protein whose expression is UV-sensitive.

作者信息

Uchida H, Suzuki L, Anai T, Doi K, Takano H, Yamashita H, Oka T, Kawano S, Tomizawa K I, Kawazu T, Kuroiwa H, Kuroiwa T

机构信息

Institute of Biological Sciences, University of Tsukuba, Tsukuba 305-8577, Japan.

出版信息

Curr Genet. 1999 Oct;36(4):232-40. doi: 10.1007/s002940050495.

DOI:10.1007/s002940050495

PMID:10541861

Abstract

Uniparental inheritance of the chloroplast genome has been observed in a wide variety of green plants. In Chlamydomonas this phenomenon, which can be selectively inhibited by UV irradiation of mt(+) gametes, has been shown cytologically to be due to the preferential degradation of mt(-)-derived chloroplast nucleoids in young zygotes. The zygote-specific pair of zys1 genes, zys1A and zys1B, is expressed earliest among five genes isolated from a "10-min" zygote library. We report here that the ZYS1 protein, which is encoded by the invertedly duplicated zys1 gene, accumulates in zygotes and is localized in nuclei. In addition, when mt(+) gametes (but not mt(-) gametes) are UV-irradiated before mating, only very limited accumulation of ZYS1 protein can be detected in the resulting zygotes.

摘要

在各种各样的绿色植物中都观察到了叶绿体基因组的单亲遗传现象。在衣藻中，这种现象可通过对mt（+）配子进行紫外线照射而被选择性抑制，从细胞学角度来看，这是由于年轻合子中mt（-）来源的叶绿体类核优先降解所致。合子特异性的zys1基因对，即zys1A和zys1B，在从一个“10分钟”合子文库中分离出的五个基因中最早表达。我们在此报告，由反向重复的zys1基因编码的ZYS1蛋白在合子中积累并定位于细胞核。此外，当mt（+）配子（而非mt（-）配子）在交配前受到紫外线照射时，在产生的合子中只能检测到非常有限的ZYS1蛋白积累。