大肠杆菌ada调节子在稳定期的表达：alkA转录受rpoS依赖性负调控的证据。
Expression of the Escherichia coli ada regulon in stationary phase: evidence for rpoS-dependent negative regulation of alkA transcription.
作者信息
Landini P, Busby S J
机构信息
Swiss Federal Institute for Environmental Science and Technology (EAWAG), 8600 Dübendorf, Switzerland.
出版信息
J Bacteriol. 1999 Nov;181(21):6836-9. doi: 10.1128/JB.181.21.6836-6839.1999.
Abstract
The Escherichia coli Ada protein activates sigma(70)-dependent transcription at three different promoters (ada, aidB, and alkA) in response to alkylation damage of DNA. During stationary phase, however, the methylated form of Ada shuts off expression of alkA; this repression is specific for sigma(S)-dependent transcription. Thus, at the alkA promoter, the Ada protein can act as both a positive and negative modulator of the adaptive response to alkylation damage, depending on the cell's physiological state.
摘要
大肠杆菌Ada蛋白可响应DNA的烷基化损伤，在三个不同启动子（ada、aidB和alkA）处激活依赖于σ⁷⁰的转录。然而，在稳定期，Ada的甲基化形式会关闭alkA的表达；这种抑制作用对依赖于σˢ的转录具有特异性。因此，在alkA启动子处，根据细胞的生理状态，Ada蛋白既可以作为对烷基化损伤适应性反应的正调节因子，也可以作为负调节因子。
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