Spångberg K, Goobar-Larsson L, Wahren-Herlenius M, Schwartz S
Department of Medical Biochemistry and Microbiology, Biomedical Center, Uppsala University, Sweden.
J Hum Virol. 1999 Sep-Oct;2(5):296-307.
To identify cellular factors in human liver that interact with hepatitis C virus (HCV) 5' and 3' untranslated regions (UTRs) and therefore possibly are involved in the regulation of HCV transcription or translation.
We prepared cytoplasmic extracts from human liver biopsy samples and fractionated cellular factors on ion exchange columns. The various fractions from ion exchange columns were subjected to ultraviolet (UV) cross-linking with radiolabeled HCV 5' and 3' UTR RNA probes.
Two major 45- and 46-kd proteins that interacted specifically with the HCV 5' and 3' UTR were identified. Using Western blot and immunoprecipitation, these proteins were identified as the La antigen.
Our results demonstrate that the La protein from human liver biopsy cells interacts specifically with the U-rich region in the positive strand of the HCV 3' UTR.
鉴定人肝脏中与丙型肝炎病毒（HCV）5′和3′非翻译区（UTR）相互作用、因此可能参与HCV转录或翻译调控的细胞因子。
我们从人肝活检样本中制备细胞质提取物，并在离子交换柱上分离细胞因子。离子交换柱的各个组分用放射性标记的HCV 5′和3′UTR RNA探针进行紫外线（UV）交联。
鉴定出两种与HCV 5′和3′UTR特异性相互作用的主要45 kDa和46 kDa蛋白质。通过蛋白质免疫印迹法和免疫沉淀法，这些蛋白质被鉴定为La抗原。
我们的结果表明，人肝活检细胞中的La蛋白与HCV 3′UTR正链中的富含U区域特异性相互作用。
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