通过Dazl直系同源物Boule对减数分裂Cdc25蛋白Twine进行转录后调控。

Post-transcriptional regulation of the meiotic Cdc25 protein Twine by the Dazl orthologue Boule.

作者信息

Maines J Z, Wasserman S A

机构信息

Center for Molecular Genetics, University of California at San Diego, La Jolla 92093-0634, USA.

出版信息

Nat Cell Biol. 1999 Jul;1(3):171-4. doi: 10.1038/11091.

DOI:10.1038/11091

PMID:10559904

Abstract

Boule, a Drosophila orthologue of the vertebrate Dazl fertility factors, is a testis-specific regulator of meiotic entry and germline differentiation. Mutations inactivating either Boule, which is an RNA-binding protein, or Twine, which is a Cdc25-type phosphatase, block meiotic entry in males. Here we show that twine and boule interact genetically. We also find that protein expression from twine messenger RNA correlates with cytoplasmic accumulation of Boule and is markedly reduced by boule mutations. Remarkably, heterologous expression of Twine rescues the boule meiotic-entry defect, indicating that the essential function of Boule at the transition from G2 to M phase during meiosis is in the control of Twine translation.

摘要

Boule是脊椎动物Dazl生育因子在果蝇中的同源物，是减数分裂起始和生殖系分化的睾丸特异性调节因子。使作为RNA结合蛋白的Boule或作为Cdc25型磷酸酶的Twine失活的突变会阻止雄性减数分裂的起始。在这里，我们表明twine和boule在遗传上相互作用。我们还发现，来自twine信使RNA的蛋白质表达与Boule的细胞质积累相关，并且在boule突变时显著降低。值得注意的是，Twine的异源表达挽救了boule减数分裂起始缺陷，表明Boule在减数分裂期间从G2期到M期转变过程中的基本功能是控制Twine的翻译。