[新开发的MB氧化还原系统与分枝杆菌生长指示管（MGIT）及2%小川鸡蛋培养基用于临床标本中分枝杆菌回收的比较]

[Comparison of the newly developed MB redox system with mycobacteria growth indicator tube (MGIT) and 2% Ogawa egg media for recovery of mycobacteria in clinical specimens].

作者信息

Abe C, Hirano K, Wada M, Tsubura E, Yamanaka M, Aoyagi T, Osumi M, Takeda M, Kurashima A, Yoneyama A, Okuzumi K

机构信息

Research Institute of Tuberculosis, Japan Anti-Tuberculosis Association, Tokyo Japan.

出版信息

Kekkaku. 1999 Oct;74(10):707-13.

PMID:10565130

Abstract

The rate of recovery and the mean time to detection of mycobacteria in clinical specimens were determined in a newly-developed MB Redox system based on liquid medium, and the results were compared with those of MGIT and 2% Ogawa egg media. From 587 sputum specimens processed, totally 203 mycobacterial isolates were detected, of which 177 (87.2%) with MB Redox, 185 (91.1%) with MGIT and 133 (65.6%) with 2% Ogawa medium. The difference in the percentages of positive cultures between either of the two liquid media and 2% Ogawa medium was significant (p < 0.0001). The mean time to detection of the Mycobacterium tuberculosis complex was 17.5 days with MB Redox, 18.7 days with MGIT, and 26.2 days with 2% Ogawa medium. The contamination rates were 1.5, 1.7, and 4.1% for MB Redox, MGIT, and 2% Ogawa medium, respectively. In conclusion, both MB Redox and MGIT systems, based on liquid medium, are more efficient than 2% Ogawa medium for the recovery of mycobacteria in clinical specimens.

摘要

在一种新开发的基于液体培养基的MB氧化还原系统中，测定了临床标本中分枝杆菌的回收率和平均检测时间，并将结果与MGIT和2%小川鸡蛋培养基的结果进行了比较。在处理的587份痰标本中，共检测到203株分枝杆菌分离株，其中MB氧化还原法检测到177株（87.2%），MGIT法检测到185株（91.1%），2%小川培养基检测到133株（65.6%）。两种液体培养基与2%小川培养基之间阳性培养物百分比的差异具有统计学意义（p<0.0001）。结核分枝杆菌复合群的平均检测时间，MB氧化还原法为17.5天，MGIT法为18.7天，2%小川培养基为26.2天。MB氧化还原法、MGIT法和2%小川培养基的污染率分别为1.5%、1.7%和4.1%。总之，基于液体培养基的MB氧化还原系统和MGIT系统在临床标本中分枝杆菌的回收方面比2%小川培养基更有效。