Marmolejo-Valencia A, Nishioka Y, Moreno-Mendoza N, Merchant-Larios H
Instituto de Investigaciones Biomédicas, Universidad Nacional Autonoma de Mexico, 04510 México, D.F., Mexico.
Biol Reprod. 1999 Dec;61(6):1426-30. doi: 10.1095/biolreprod61.6.1426.
When the Y chromosome of Mus musculus domesticus (Y(TIR)) was introduced onto the C57BL/6J (B6) mouse background, testis development was impaired and half of the XY progeny (Y(TIR).B6) developed a female phenotype. Y(TIR).B6 fetal ovaries showed massive death of medullary oocytes and, after birth, produced abnormal levels of steroid hormones, exhibited irregular estrous cycles, and failed to become fertile. In this study we examined whether alterations during perinatal development observed in Y(TIR).B6 ovaries permanently impaired the establishment of the hypothalamus-pituitary-ovary axis (HPOa). B6 fetal and postnatal ovaries at different stages (fetal, infantile, or adult) were transplanted orthotopically (to the ovarian bursa) to either ovariectomized B6 normal females or Y(TIR).B6 sex-reversal females. Percentage of pregnancy, litter size, and capacity to feed pups were recorded. Reciprocally, XY(TIR).B6 ovaries were orthotopically transplanted into B6 females. After crossing with fertile males, several Y(TIR).B6 sex-reversal females with B6 ovarian transplants at all ages became pregnant, had offspring, and fed their pups. On the other hand, none of the B6 female hosts with XY(TIR) ovaries became pregnant. Results demonstrated that Y(TIR).B6 sex-reversal females maintain a functional HPOa and that their failure to reproduce is primarily due to an ovarian defect.
当将小家鼠的Y染色体（Y(TIR)）导入C57BL/6J（B6）小鼠背景时，睾丸发育受损，一半的XY后代（Y(TIR).B6）出现雌性表型。Y(TIR).B6胎鼠卵巢显示髓质卵母细胞大量死亡，出生后，其产生的类固醇激素水平异常，发情周期不规律，且无法生育。在本研究中，我们检查了在Y(TIR).B6卵巢中观察到的围产期发育变化是否会永久性损害下丘脑-垂体-卵巢轴（HPOa）的建立。将不同阶段（胎儿期、婴儿期或成年期）的B6胎鼠和出生后卵巢原位移植（至卵巢囊）到卵巢切除的B6正常雌性小鼠或Y(TIR).B6性反转雌性小鼠体内。记录怀孕百分比、窝仔数和哺育幼崽的能力。反之，将XY(TIR).B6卵巢原位移植到B6雌性小鼠体内。与可育雄性交配后，所有年龄段接受B6卵巢移植的几只Y(TIR).B6性反转雌性小鼠都怀孕、产仔并哺育幼崽。另一方面，接受XY(TIR)卵巢移植的B6雌性宿主均未怀孕。结果表明，Y(TIR).B6性反转雌性小鼠维持着功能性的HPOa，其无法繁殖主要是由于卵巢缺陷。
