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使用有机溶剂将胆固醇氧化酶固定在福尔马林中。

Immobilization of cholesterol oxidase on Formvar using organic solvents.

作者信息

Kumar H, Kumar A, Kumari P, Jyotirmai S, Tulsani N B

机构信息

Centre for Biochemical Technology, Mall Road, Delhi-110007, India.

出版信息

Biotechnol Appl Biochem. 1999 Dec;30(3):231-3.

Abstract

The present study describes a new immobilization support for the preparation of enzyme membrane in the presence of organic solvents. The support was composed of Formvar solubilization in organic solvents with enzyme. More than 90% of the enzyme was immobilized on the membrane. The membrane was prepared by mixing 4% Formvar in organic solvents and 1% cholesterol oxidase was immobilized by entrapment technique. Practically no leaching of the entrapped enzyme was observed. The resultant immobilized enzyme membrane was stored at 4 degrees C for 10 days without losing its activity. The pH and temperature stabilities were greater than those of the native enzyme. The immobilized enzyme membrane has a long life due to its hydrophobic nature, as compared with other membranes.

摘要

本研究描述了一种在有机溶剂存在下制备酶膜的新型固定化载体。该载体由在有机溶剂中与酶溶解的福尔马林组成。超过90%的酶被固定在膜上。通过将4%的福尔马林在有机溶剂中混合制备膜,并通过包埋技术固定1%的胆固醇氧化酶。实际上未观察到包埋酶的渗漏。所得固定化酶膜在4℃下储存10天而不失活。其pH稳定性和温度稳定性均高于天然酶。与其他膜相比,固定化酶膜因其疏水性质而具有较长的使用寿命。

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