越南TT病毒感染与HIV和HCV感染风险行为之间不存在关联。

Lack of association between acquisition of TT virus and risk behavior for HIV and HCV infection in Vietnam.

作者信息

Nerurkar V R, Woodward C L, Nguyen H T, DeWolfe Miller F, Tashima L T, Zalles-Ganley A, Chua P K, Peterson J E, Chi P K, Hoang L T, Detels R, Yanagihara R

机构信息

Retrovirology Research Laboratory, Pacific Biomedical Research Center, University of Hawaii at Manoa, Honolulu, Hawaii 96816, USA.

出版信息

Int J Infect Dis. 1999 Summer;3(4):181-5. doi: 10.1016/s1201-9712(99)90021-8.

DOI:10.1016/s1201-9712(99)90021-8

PMID:10575145

Abstract

BACKGROUND

The search for the cause of chronic hepatitis among individuals with non-A to G hepatitis has led to the discovery of a post-transfusion hepatitis-related DNA virus, designated TT virus (TTV), which, based on viral sequences, belongs to a new virus family. The principal modes of infection with TTV are poorly understood, and its role in human immunodeficiency virus type 1 (HIV-1) infection is unclear.

OBJECTIVE

To determine if injection drug use (IDU) and high-risk heterosexual activity (HRHA), principal modes of acquiring HIV-1 infection, place individuals at greater risk of acquiring TTV.

METHODS

The authors analyzed DNA, extracted from sera or filter paper-blotted whole blood, obtained during August 1997 and June 1998 from 324 Vietnamese (148 male; 176 female), for TTV sequences by hot-start, heminested polymerase chain reaction.

RESULTS

Prevalence of TTV viremia was similar among individuals engaging in IDU or HRHA (23.4% vs. 20.2%; P > 0.5), with no age- or gender-specific differences. No association was found between TTV viremia and co-infection with HIV-1 or hepatitis C virus (HCV). Phylogenetic analysis of 30 TTV sequences revealed two distinct genotypes and four subtypes that did not segregate according to gender, HIV-1 and HCV risk behaviors, or geographic residence.

CONCLUSIONS

Among HIV-1- or HCV-infected Vietnamese, who presumably acquired their infection by either the parenteral or nonparenteral route, the data indicate no clear association between acquisition of TTV infection and risk behavior for HIV-1 or HCV infection, suggesting that the usual route of TTV transmission in Vietnam is other than parenteral or sexual.

摘要

背景

在非甲至庚型肝炎患者中寻找慢性肝炎病因的过程中，发现了一种与输血后肝炎相关的DNA病毒，命名为TT病毒（TTV），根据病毒序列，它属于一个新的病毒家族。人们对TTV的主要感染方式了解甚少，其在1型人类免疫缺陷病毒（HIV-1）感染中的作用尚不清楚。

目的

确定注射吸毒（IDU）和高危异性性行为（HRHA）这两种感染HIV-1的主要方式是否会使个体感染TTV的风险更高。

方法

作者通过热启动半巢式聚合酶链反应，分析了1997年8月至1998年6月期间从324名越南人（148名男性；176名女性）的血清或滤纸片干血中提取的DNA，以检测TTV序列。

结果

在注射吸毒者或有高危异性性行为者中，TTV病毒血症的患病率相似（23.4%对20.2%；P>0.5），无年龄或性别差异。未发现TTV病毒血症与HIV-1或丙型肝炎病毒（HCV）合并感染之间存在关联。对30个TTV序列的系统发育分析揭示了两种不同的基因型和四种亚型，它们并未根据性别、HIV-1和HCV风险行为或地理居住地进行区分。