Jourdan A D, Stauffer G V
Department of Microbiology, University of Iowa, Iowa City, IA 52242, USA.
FEMS Microbiol Lett. 1999 Dec 15;181(2):307-12. doi: 10.1111/j.1574-6968.1999.tb08860.x.
Several LysR-type transcriptional regulators have been shown to require the carboxy-terminal domain of the alpha subunit (alphaCTD) of RNA polymerase to activate their target genes. We show here that GcvA, a LysR-type protein, also uses the alphaCTD to activate the Escherichia coli gcvTHP operon. Amino acid residues in the alphaCTD important for GcvA-dependent activation, however, have no effect on GcvA-mediated repression of the operon.
已有研究表明,几种赖斯氏菌属转录调节因子需要RNA聚合酶α亚基的羧基末端结构域(αCTD)来激活其靶基因。我们在此表明,赖斯氏菌属蛋白GcvA也利用αCTD来激活大肠杆菌的gcvTHP操纵子。然而,αCTD中对GcvA依赖性激活很重要的氨基酸残基对GcvA介导的该操纵子的抑制没有影响。