鸡胚胎学中的“惊人”进展：电穿孔与卵内基因表达。

'Shocking' developments in chick embryology: electroporation and in ovo gene expression.

作者信息

Itasaki N, Bel-Vialar S, Krumlauf R

机构信息

Laboratory of Developmental Neurobiology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK.

出版信息

Nat Cell Biol. 1999 Dec;1(8):E203-7. doi: 10.1038/70231.

DOI:10.1038/70231

PMID:10587659

Abstract

Efficient gene transfer by electroporation of chick embryos in ovo has allowed the development of new approaches to the analysis of gene regulation, function and expression, creating an exciting opportunity to build upon the classical manipulative advantages of the chick embryonic system. This method is applicable to other vertebrate embryos and is an important tool with which to address cell and developmental biology questions. Here we describe the technical aspects of in ovo electroporation, its different applications and future perspectives.

摘要

通过对鸡胚进行卵内电穿孔实现高效基因转移，这使得分析基因调控、功能和表达的新方法得以发展，为在鸡胚系统经典操作优势的基础上进一步拓展创造了令人兴奋的机会。该方法适用于其他脊椎动物胚胎，是解决细胞和发育生物学问题的重要工具。在此，我们描述卵内电穿孔的技术要点、其不同应用及未来展望。