来自荚膜红细菌和大肠杆菌的核糖核酸酶E在多顺反子puf mRNA内的上下文和序列依赖性体内切割方面存在差异。
RNase E enzymes from rhodobacter capsulatus and Escherichia coli differ in context- and sequence-dependent in vivo cleavage within the polycistronic puf mRNA.
作者信息
Heck C, Evguenieva-Hackenberg E, Balzer A, Klug G
机构信息
Institut für Mikrobiologie und Molekularbiologie, D-35392 Giessen, Germany.
出版信息
J Bacteriol. 1999 Dec;181(24):7621-5. doi: 10.1128/JB.181.24.7621-7625.1999.
Abstract
The 5' pufQ mRNA segment and the pufLMX mRNA segment of Rhodobacter capsulatus exhibit different stabilities. Degradation of both mRNA segments is initiated by RNase E-mediated endonucleolytic cleavage. While Rhodobacter RNase E does not discriminate between the different sequences present around the cleavage sites within pufQ and pufL, Escherichia coli RNase E shows preference for the sequence harboring more A and U residues.
摘要
荚膜红细菌的5' pufQ mRNA片段和pufLMX mRNA片段表现出不同的稳定性。这两个mRNA片段的降解均由核糖核酸酶E介导的内切核酸酶切割引发。虽然荚膜红细菌核糖核酸酶E对pufQ和pufL内切割位点周围存在的不同序列没有区分，但大肠杆菌核糖核酸酶E对含有更多A和U残基的序列表现出偏好。
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