去负荷大鼠比目鱼肌单根肌纤维中肌球蛋白重链mRNA和蛋白异构体的变化。

Changes in myosin heavy chain mRNA and protein isoforms in single fibers of unloaded rat soleus muscle.

作者信息

Stevens L, Gohlsch B, Mounier Y, Pette D

机构信息

Faculty of Biology, University of Konstanz, D-78457, Konstanz, Germany.

出版信息

FEBS Lett. 1999 Dec 10;463(1-2):15-8. doi: 10.1016/s0014-5793(99)01596-3.

DOI:10.1016/s0014-5793(99)01596-3

PMID:10601629

Abstract

Changes in myosin heavy chain (MHC) mRNA and protein isoforms were investigated in single fibers from rat soleus muscle unloaded by hindlimb suspension for 4 and 7 days. Dramatic changes were seen after 4 days, when all fibers co-expressed slow and fast MHC mRNAs. Most fibers contained mRNAs for MHCIbeta, MHCIIa, MHCIId(x), and MHCIIb. The up-regulation of the fast isoforms was only partially transmitted to the protein level. Atypical combinations of MHC mRNA isoforms, which deviated from the 'next-neighbor rule', were frequent in fibers from unloaded soleus. These atypical combinations increased with time and were not observed in the controls. The results suggest that hindlimb suspension elicits in soleus muscle pronounced perturbations in the expression of MHC isoforms by disrupting transcriptional and translational activities.

摘要

对后肢悬吊4天和7天的大鼠比目鱼肌单根纤维中肌球蛋白重链（MHC）mRNA和蛋白质异构体的变化进行了研究。4天后出现了显著变化，此时所有纤维共同表达慢速和快速MHC mRNA。大多数纤维含有MHCIβ、MHCIIa、MHCIId（x）和MHCIIb的mRNA。快速异构体的上调仅部分传递到蛋白质水平。偏离“相邻规则”的MHC mRNA异构体的非典型组合在卸载比目鱼肌的纤维中很常见。这些非典型组合随时间增加，在对照组中未观察到。结果表明，后肢悬吊通过破坏转录和翻译活动，在比目鱼肌中引起MHC异构体表达的明显扰动。