墨西哥人群中六个扩增片段长度多态性（Amp-FLPs，即D1S80、APO-B、VWA、TH01、CSF1PO和HPRTB）的等位基因频率分布。

Allele frequency distributions of six Amp-FLPS (D1S80, APO-B, VWA, TH01, CSF1PO and HPRTB) in a Mexican population.

作者信息

Rangel-Villalobos H, Rivas F, Torres-Rodríguez M, Jaloma-Cruz A R, Gallegos-Arreola M P, López-Satow J, Cantú J M, Figuera L E

机构信息

División de Genética, Centro de Investigación Biomédica de Occidente (CIBO-IMSS), Guadalajara, Jalisco, Mexico.

出版信息

Forensic Sci Int. 1999 Nov 1;105(2):125-9. doi: 10.1016/s0379-0738(99)00129-2.

DOI:10.1016/s0379-0738(99)00129-2

PMID:10605081

Abstract

Six amplified fragment length polymorphisms or Amp-FLPs, two VNTRs (D1S80 and APO-B) and four STRs (VWA, TH01, CSF1PO and HPRTB), were typed in a Mexican population of the Jalisco state by means of non-denaturing polyacrylamide gel electrophoresis (native PAGE) in standard gel units and silver staining. Genotype distribution was in agreement with Hardy-Weinberg expectations (HWE) for all six markers. Heterozygosity ranged from 70.6 to 83.5%, the cumulated chance of exclusion (CE) and power of discrimination (PD) were 99.4 and 99.99%, respectively. STRs and D1S80 allele frequency distributions (AFD) were similar (P > 0.05) to U.S. Hispanics, but different to U.S. Caucasians and African-Americans. APO-B exhibited similarities with White Brazilians, Spaniards, but differences (P < 0.05) with Amerindian and Black Brazilians.

摘要

通过在标准凝胶装置中采用非变性聚丙烯酰胺凝胶电泳（天然聚丙烯酰胺凝胶电泳）和银染法，对哈利斯科州的一个墨西哥人群体进行了6种扩增片段长度多态性（Amp-FLP）分析，即2种可变数目串联重复序列（VNTR，分别为D1S80和APO-B）和4种短串联重复序列（STR，分别为VWA、TH01、CSF1PO和HPRTB）的分型。所有6种标记的基因型分布均符合哈迪-温伯格平衡预期（HWE）。杂合度范围为70.6%至83.5%，累积排除概率（CE）和鉴别能力（PD）分别为99.4%和99.99%。STR和D1S80的等位基因频率分布（AFD）与美国西班牙裔相似（P>0.05），但与美国白种人和非裔美国人不同。APO-B与巴西白人、西班牙人表现出相似性，但与巴西印第安人和巴西黑人存在差异（P<0.05）。