传染性猫泡沫病毒基因组的构建：猫抗血清不能交叉中和具有血清型特异性Env序列的猫泡沫病毒嵌合体。

Construction of infectious feline foamy virus genomes: cat antisera do not cross-neutralize feline foamy virus chimera with serotype-specific Env sequences.

作者信息

Zemba M, Alke A, Bodem J, Winkler I G, Flower R L, Pfrepper K, Delius H, Flügel R M, Löchelt M

机构信息

Forschungsschwerpunkt Angewandte Tumorvirologie, Deutsches Krebsforschungszentrum, Heidelberg, 69009, Germany.

出版信息

Virology. 2000 Jan 5;266(1):150-6. doi: 10.1006/viro.1999.0037.

DOI:10.1006/viro.1999.0037

PMID:10612669

Abstract

Full-length genomes of the feline foamy virus (FFV or FeFV) isolate FUV were constructed. DNA clone pFeFV-7 stably directed the expression of infectious FFV progeny virus indistinguishable from wild-type, uncloned FFV isolate FUV. The env and bel 1 genes of pFeFV-7 were substituted for by corresponding sequences of the FFV serotype 951 since previous studies implicated a defined part of FFV Env protein as responsible for serotype-specific differences in serum neutralization (I. G. Winkler, R. M. Flügel, M. Löchelt, and R. L. P. Flower, 1998. Virology 247: 144-151). Recombinant virus derived from chimeric plasmid pFeFV-7/951 containing the hybrid env gene and the parental clone pFeFV-7 were used for neutralization studies. By means of a rapid titration assay for FFV infectivity, we show that progeny virus derived from plasmid pFeFV-7 was neutralized by FUV- but not by 951-specific antisera, whereas pFeFV-7/951-derived chimeric virus was neutralized by 951-specific antisera only. Both recombinant proviruses will be useful for repeated delivery of foreign genes for therapeutic gene applications into cats.

摘要

构建了猫泡沫病毒（FFV 或 FeFV）分离株 FUV 的全长基因组。DNA 克隆 pFeFV - 7 稳定地指导了与野生型、未克隆的 FFV 分离株 FUV 无法区分的感染性 FFV 子代病毒的表达。由于先前的研究表明 FFV Env 蛋白的特定部分负责血清中和中的血清型特异性差异（I.G. 温克勒、R.M. 弗吕格尔、M. 勒歇尔特和 R.L.P. 弗劳尔，1998 年。《病毒学》247: 144 - 151），因此用 FFV 血清型 951 的相应序列替换了 pFeFV - 7 的 env 和 bel 1 基因。源自含有杂交 env 基因的嵌合质粒 pFeFV - 7/951 和亲本克隆 pFeFV - 7 的重组病毒用于中和研究。通过一种针对 FFV 感染性的快速滴定测定法，我们表明源自质粒 pFeFV - 7 的子代病毒被 FUV 特异性抗血清中和，但不被 951 特异性抗血清中和，而源自 pFeFV - 7/951 的嵌合病毒仅被 951 特异性抗血清中和。这两种重组前病毒都将有助于将用于治疗性基因应用的外源基因重复递送至猫体内。