通过对Taq DNA聚合酶修饰的P1质粒DNA的短片段进行PCR扩增和TA克隆来进行基因步移。 - Suppr

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超能文献

Gene walking by PCR amplification of short fragments from Taq DNA polymerase--modified P1 plasmid DNA and TA cloning.

作者信息

Baury B, Masson D, Lustenberger P, Denis M G

机构信息

Institut de Biologie Nantes, France.

出版信息

Biotechniques. 1999 Dec;27(6):1118-20, 1122. doi: 10.2144/99276bm06.

DOI:10.2144/99276bm06

PMID:10631487

Abstract

摘要

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Gene walking by PCR amplification of short fragments from Taq DNA polymerase--modified P1 plasmid DNA and TA cloning.

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相似文献

Gene walking by PCR amplification of short fragments from Taq DNA polymerase--modified P1 plasmid DNA and TA cloning.

Biotechniques. 1999 Dec;27(6):1118-20, 1122. doi: 10.2144/99276bm06.

Directional enrichment of directly cloned PCR products.

Biotechniques. 2005 Jul;39(1):40, 42, 44, 46. doi: 10.2144/05391BM03.

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Cold Spring Harb Protoc. 2021 Jun 1;2021(6):2021/6/pdb.prot101303. doi: 10.1101/pdb.prot101303.

Distinct combination of purification methods dramatically improves cohesive-end subcloning of PCR products.

Biotechniques. 1998 Apr;24(4):578-80. doi: 10.2144/98244bm13.

Digestion of terminal restriction endonuclease recognition sites on PCR products.

Biotechniques. 1998 Apr;24(4):582-4. doi: 10.2144/98244bm15.

Cloning Polymerase Chain Reaction (PCR) Products: Making T Vectors.

Cold Spring Harb Protoc. 2021 Jun 1;2021(6):2021/6/pdb.prot101295. doi: 10.1101/pdb.prot101295.

A highly efficient technique for cloning of refractory DNA fragments and polymerase chain reaction products.

Anal Biochem. 1994 Aug 1;220(2):426-8. doi: 10.1006/abio.1994.1362.

Universal TA cloning.

Curr Issues Mol Biol. 2000 Jan;2(1):1-7.

Thermostable ligase-mediated incorporation of mutagenic oligonucleotides during PCR amplification.

Methods Mol Biol. 1997;67:189-95. doi: 10.1385/0-89603-483-6:189.

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Gene walking by PCR amplification of short fragments from Taq DNA polymerase--modified P1 plasmid DNA and TA cloning.

作者信息

Baury B, Masson D, Lustenberger P, Denis M G

机构信息

Institut de Biologie Nantes, France.

出版信息

Biotechniques. 1999 Dec;27(6):1118-20, 1122. doi: 10.2144/99276bm06.

DOI:10.2144/99276bm06

PMID:10631487

Abstract

摘要